Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE2 and Th2 Cytokines
Figure 6
Th2 cytokines and PGE2 inhibit eosinophil degranulation. Purified human eosinophils were pretreated with vehicle, IL-4 (10 ng/mL), IL-5 (10 ng/mL), IL-13 (10 ng/mL), and PGE2 (10−6 M) for 1 or 2 hours and then stimulated with C5a (300 nM) for 30 min at 37°C. The release of EPO activity into supernatants was determined by photometry. Data were expressed as a percentage of the maximal control response (300 nM) and are shown as the mean ± SD; –9; , versus C5a alone (vehicle).
