Journal of Immunology Research / 2012 / Article / Fig 2

Research Article

ELISA-Based Measurement of Antibody Responses and PCR-Based Detection Profiles Can Distinguish between Active Infection and Early Clearance of Borrelia burgdorferi

Figure 2

Antibodies produced against live and killed spirochetes recognize unique Bb antigens. Sera were collected from individual mice 4 weeks after injection with a single dose of either BSK II medium (lanes 2-3), 5 × 107 killed Bb (lanes 4–9), 250 live Bb (lanes 10–12), or 5 × 104 live Bb (lanes 14–16); one group initially received 105 killed Bb + CFA, with a boost 3 weeks later with 105 killed Bb + IFA, and sera collected 3 weeks later (lane 13). All sera were diluted either 1 : 25 (lanes 2-3 and 7–9) or 1 : 500 before using to immunoblot membranes containing electrophoresed Bb. A mAb specific for Bb OspA, H5332 [49], was included as a marker for OspA reactivity (lane 1).

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