Clinical Isolates of Mycobacterium tuberculosis Differ in Their Ability to Induce Respiratory Burst and Apoptosis in Neutrophils as a Possible Mechanism of Immune Escape
Figure 3
CD11b, CD66b, and p-p38 MAPK expression in PMN induced by Mtb strains. PMNs (3 ×106/mL) were cultured in media alone (control, —) or stimulated with Mtb strains at 1 : 2 Mtb : PMN ratio for 3 h. (a) and (b) Surface expression of activation markers was evaluated by flow cytometry and results are expressed as media ± SEM of MFI (). Statistical differences: CD11b and CD66b: control (—) versus H37Rv, LAM, Ra and H: ; control versus M: ; LAM and Ra versus other strains: ; (c) PMNs (3 × 106/mL) were cultured in media alone (control, —) or stimulated with Mtb strains at 1 : 2 Mtb : PMN ratio for 1 h. Intracellular activated form of p38 (p-p38) was measured by flow cytometry. Results are expressed as media ± SEM (). Statistical differences: control versus H37Rv, LAM, Ra, and H: ; LAM and Ra versus other strains: .
