Immunological and Nonimmunological Effects of Indoleamine 2,3-Dioxygenase on Breast Tumor Growth and Spontaneous Metastasis Formation
Table 2
Ido1 and the cell cycle genes expression in mouse breast tumor cells.
Fold changes in cell cycle genes expression
4T1/Ido1+ versus 4T1/Ido1−
NT-5/Ido1− versus NT-5/Ido1+
G1 phase and G1/S transition
1
CAMK2a
1.3
5.4
2
GPR132
4.8
1.8s
3
ITGB1
− 17.6
1.8
4
PPP2r3a
5.1
2.9
5
PPP3ca
− 4.2
2.1
6
SKP2
− 3.8
1.5
S phase and DNA replication
7
DNAJC2
7.7
1.5
8
MKI67
8.8
2.3
9
MRE11a
− 4.9
1.2
10
MSH2
− 3.3
1.5
11
PCNA
− 5.4
1.1
12
RAD17
− 6.1
1.8
13
RAD51
− 5.2
1.3
M phase
14
BRCA2
− 7.1
1.8
15
CCNA1
19.1
4.4
16
CCNB1
− 10.9
− 6.8
17
CDC25a
− 3.1
1.9
18
NEK2
− 4.4
1.1
19
NPM2
− 1.7
3.1
20
PRM1
10.0
1.6
21
RAD21
3.3
2.8
22
SMC1a
8.6
1.4
23
STAG1
− 5.8
3.0
G2 phase and G2/M transition
24
CHEK1
− 31.2
1.3
25
DNAJC2
− 7.7
1.5
Cell cycle checkpoint; cell cycle arrest
26
BRCA2
− 7.1
1.8
27
CDK5rap1
− 6.5
1.2
28
CDKN1a(p21)
11.6
2.0
29
CDKN1b(p27)
− 6.8
1.0
30
CDKN2a(p16)
9.9
2.4
31
CASP3
− 5.6
1.4
32
CHEK1
− 31.2
1.3
33
DDIT3
− 1.8
14.3
34
DST
− 11.2
7.3
35
HUS1
− 7.7
1.0
36
INHA
10.0
5.0
37
MACF1
− 8.7
3.1
38
NOTCH2
− 1.8
3.1
39
PKD1
− 2.0
3.5
40
PMP22
− 1.9
6.1
41
SFN
12.0
− 7.0
Regulation of the cell cycle
42
ABL1
− 3.4
1.3
43
ATM
− 18.3
1.7
44
BRCA1
− 12.5
1.3
45
CCNC
− 11.0
1.0
46
PBL1
− 8.9
1.5
47
E2F3
3.3
− 1.2
48
TRP63(p63)
− 27.3
6.8
4T1: stable clone with shRNA-negative control vector pGeneClip/Neomycin; 4T1/ Ido1−: stable clone with shRNA for IDO. NT-5: stable clone with control vector pRc/CMV/Neomycin; NT-5/Ido1+: stable clone with cDNA IDO.
*A positive value (normal font) indicates fold of gene upregulation; a negative value (bold) indicates fold of gene downregulation. Genes which were found up/downregulated more than 3-fold at least in the one of the cell line are presented.
