Interleukin-12p70 Expression by Dendritic Cells of HIV-1-Infected Patients Fails to Stimulate gag-Specific Immune Responses
Figure 1
Production of pro- and anti-inflammatory cytokines by DCs modulated to express IL-12p70. (a) IL-12 secretion by DCs electroporated with IL-12p70-encoding mRNA. DCs were examined after incubation with a proinflammatory cytokine cocktail consisting of TNF-α and PGE2 and electroporated with mRNA encoding IL-12p70 or with IL-12p70-encoding mRNA in combination with hHxB-2 gag-encoding mRNA. As a control mock-electroporated mature DCs were examined. DC culture supernatant was collected 24 h, 48 h, and 96 h after electroporation. Results are shown as mean ± standard deviation (). (b) IL-12 secretion by immune-modulated DCs. DCs were examined after incubation with a proinflammatory cytokine cocktail consisting of TNF-α and PGE2 or activated under the same conditions supplemented with R848 and IFN-γ. As a control untreated, that is, immature DCs (iDCs) were examined. DC culture supernatant was collected 24 h after activation with proinflammatory stimuli, as well as 24 h and 72 h after a washout experiment. Results are shown as mean ± standard deviation (). (c), (d), and (e) Cytokine secretion by DCs activated under different experimental conditions and after a 24 h washout period. Values are shown for (c) IL-10, (d) IL-6, and (e) TNF-α. Results are shown as mean ± SD ().
