Journal of Immunology Research / 2012 / Article / Fig 2

Research Article

Dual Functions of the C5a Receptor as a Connector for the K562 Erythroblast-Like Cell-THP-1 Macrophage-Like Cell Island and as a Sensor for the Differentiation of the K562 Erythroblast-Like Cell during Haemin-Induced Erythropoiesis

Figure 2

Contribution of the C5aR-RP S19 oligomer system to the haemin-induced differentiation of K562 cells under monocell culture conditions (a) Time schedule for the K562 monocell culture. ((b) and (c)) K562 cells expressing EGFP-ANXA3 were observed under CLSM or TEM at several time points after the haemin induction ( ). ((d) and (e)) EGFP-ANXA3-expressing K562 and K562R cells were harvested at several time points after the haemin induction. After transferring the proteins from the SDS gel onto the membrane, the first immunoblotting was performed using the rabbit IgGs against C5aR, RP S19, and TGII (d) or ERK1/2, p38MAPK, RGS3, and actin (e) ( ).

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