Journal of Immunology Research / 2012 / Article / Fig 6

Research Article

Dual Functions of the C5a Receptor as a Connector for the K562 Erythroblast-Like Cell-THP-1 Macrophage-Like Cell Island and as a Sensor for the Differentiation of the K562 Erythroblast-Like Cell during Haemin-Induced Erythropoiesis

Figure 6

Detection of the contribution of the K562-THP-1 cell interaction to the haemin-induced terminal differentiation of K562 cells using time-lapse microscopy. (a) Time schedule for the K562 cells that were cocultured with the THP-1 cells. K562 cells expressing EGFP-ANXA3/Wild-type RP S19 (b) and EGFP-ANXA3/Gln137Asn mutant RP S19 (c) were cultured with the THP-1 macrophage-like cells for 1–3 days and monitored using the LCV110 microscope ( ).

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