HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro
Figure 1
miR-H6 effects on cell changes and viral replication in HSV-1-infected HCE cells. HCE cells were transfected miR-H6 mimics and mock, followed by infection with MOI 0.1 of HSV-1 24 h posttransfection. (a) The cellular morphological changes were imaged at 12 h and 24 h postinfection. CPE clearly increased, and many multinucleated giant cells could be seen in the cells treated with mock RNA and cells untreated with small RNA; fewer CPE and giant multinucleated cells could be seen in cells transfected with miR-H6 mimics. (b, c) Plaque assay showed that treatment with miR-H6 decreased the plaque sizes and numbers and had a statistically significant difference (). Cells were treated with miR-H6 mimics (0, 50, 100 nM) and mock RNA (mock) or without small RNA (HSV-1). The control group (Ctl) consisted of cells without virus infection and small RNA treatment. (d) Real-time PCR determined miR-H6 expression in pooled HSV-1-infected HCE cells, and miR-H6 levels were changed in a viral-titer-dependent manner (, one-way ANOVA, among MOI 0.1, 1, and 5).
