HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro
Figure 2
miR-H6 decreased ICP4 protein expression in HSV-1-infected HCE cells. HCE cells were transfected with miR-H6 mimics and mock RNA and then infected with MOI 0.1 of HSV-1 24 h posttransfection. The staining of ICP4 protein in HCE cells at 12 h p.i. (a) and at 24 h p.i. (b) TRITC labeled the antibody-stained ICP4 (red, left) and the hoechst-dyed nucleus (blue, middle). Images of ICP4 and the nucleus were merged (right), scalebar: 20 μm. (c) The ICP4 band was determined by Western blot. (d) Quantitative analysis of bands in (c). Significantly lower levels of ICP4 in HCE cells transfected with miR-H6 (, miR-H6 versus mock or HSV-1 alone).
