Reevaluating the Concept of Treating Experimental Tumors with a Mixed Bacterial Vaccine: Coley’s Toxin
Figure 3
Apoptosis assay. (a) Increased Caspase 3/7 activity in tumor cells after CT treatment, as quantified using the Promega Caspase-Glo 3/7 Assay according to the manufacturer’s instructions and measurement on a luminometer. (b) Flow cytometric sub-G1 Peak analysis following 24 and 48 hours of incubation with CT. Cells with DNA content lower than the G1 peak were considered to be apoptotic. Results show data of three separate experiments each performed in duplicates. Values are given as the mean ± SEM. (c) AsPC-1 cells show characteristic sub-G1 peaks at 24 hours after treatment (representative results out of three independent experiments).
