Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs

Figure 5

Re-exposure of orally tolerant animals to the tolerated antigen block the rise of ICAM-1 expression in lung parenchyma. Immunolocalization of ICAM-1 in granulomas and lung parenchyma using specific antibody coupled with fluorescein (green) and nuclear counterstainig with 4′6-diamidino-2-phenylindol (blue), 18 days after i.v. eggs injection. Confocal microscope images were captured with a 63X objective, and the graphs represent the green fluorescence intensity (the sum of gray values of all pixels divided by the area (in 𝜇m²)×10⁻³) of expression of ICAM-1 in the granuloma area (a–d) and in lung parenchyma (e–h) in nonimmunized mice (granuloma group, open bars), OVA immune controls (hatched bars), and OVA-orally tolerant mice (black bars). Data represent mean ± SEM of fluorescence intensity of duplicate slides (𝑛=5 mice/group).