Journal of Immunology Research / 2012 / Article / Fig 4

Research Article

Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis

Figure 4

Th17 cell-related factors expression stimulated by HMGB 1 in vitro. Th17 cell-related factors expression by HMGB 1 stimulus in vitro were detected by RT-qPCR. The CD4+T cells were isolated from mouse spleen, preactivated by anti-CD3 and anti-CD28, and then added to rHMGB 1. (a) RORγt mRNA expression levels after rHMGB1 stimulating in different time; (b) IL-17 mRNA expression leves after rHMGB 1 stimulus; (c) RORγt mRNA expression levels after rHMGB 1 stimulus; (d) IL-17 mRNA expression levels after CD4+T cells stimulated by rHMGB 1 stimulus. RT-qPCR analysis for target genes versus β-actin mRNA expression, the ratios of target genes used the control as 1. Data from 3 independent experiments were presented as means ± s.d. * versus control.

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