Notch Signaling Mediates TNF-α-Induced IL-6 Production in Cultured Fibroblast-Like Synoviocytes from Rheumatoid Arthritis
Figure 1
TNF-α stimulation induces the mRNA expression of Notch signaling molecules in fibroblast-like synoviocytes (FLS) from RA. (a) FLSs from 4 RA patients were cultured in the presence of TNF-α (10 ng/mL) for 0, 4, 8, 16, 24, or 36 h, and then analyzed for Hes-1 expression by PCR; (b) FLSs from 4 RA patients were cultured in the presence of TNF-α (10 ng/mL) for 24 h, followed by analysis of Notch receptors expression by PCR; (c) FLSs from 4 RA patients were cultured in the presence of TNF-α (10 ng/mL) for 24 h, and then analyzed for Notch ligands expression by PCR; J1: Jagged 1; J2: Jagged 2; D1: Delta-like 1; D3: Delta-like 3; D4: Delta-like 4. Results were normalized to unstimulated condition (basal value = 1). Each column is the mean ± SD. * for unstimulated group versus TNF-α-stimulated group.
