Notch Signaling Mediates TNF-α-Induced IL-6 Production in Cultured Fibroblast-Like Synoviocytes from Rheumatoid Arthritis
Figure 2
Notch activity contributes to TNF-α-induced IL-6 production of FLSs from RA. (a) FLSs from 4 RA patients were cultured in the presence of TNF-α (10 ng/mL) for 0, 4, 8, 16, 24, or 36 h, and then measured the levels of supernatant IL-6 and IL-8 by ELISA; (b) RA FLSs were cultured in the presence of TNF-α (10 ng/mL) in addition to the indicated concentration of DAPT (Notch signaling blocking reagent) for 24 h. Supernatant IL-6 and IL-8 were determined by ELISA; (c) RA FLSs were cultured in the presence of TNF-α (10 ng/mL) in addition to Delta-like 1 fusion protein (10 μg/mL) for indicated times. Supernatant IL-6 was determined by ELISA; (d) RA FLSs were cultured in the presence of TNF-α (10 ng/mL) in addition to indicated concentrations of Delta-like 1 fusion protein for 24 h. Supernatant IL-6 was determined by ELISA. Values are the mean ± SD.
