Journal of Immunology Research / 2012 / Article / Tab 1

Clinical Study

DNA Methyltransferase 3B Gene Promoter and Interleukin-1 Receptor Antagonist Polymorphisms in Childhood Immune Thrombocytopenia

Table 1

Allele types, PCR conditions, and PCR primers designed to amplify fragments harboring the polymorphic sites.

DNMT3B promoterIL-1 Ra

Type of polymorphismSingle-base C/T86-bp VNTR
Site of polymorphism Position 149 Intron 2
PCR primers
Upstream5′-TGCTGTGACAGGCAGAGCAG-3′5′-CTCAGCAACACTCCTAT-3′
Downstream5′-GGTAGCCGGGAACTCCACGG-3′5′-TCCTGGTCTGCAGGTAA-3′
DigestionAvrII
Allele size (bp)C: 380I: 410
II: 240
T: III: 325
IV: 500