Research Article

Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice

Table 1

B. melitensis 16 M OMVs proteins identified by 1D-SDS-PAGE coupled to LC-MS/MS.

ProteinB. melitensis denominationMolecular weight (kDa)Motif, subcellular localizationClosest ortholog, other than in Brucellae (% similarity)

Bacterial surface antigenBMEI083085.90Surface antigen, OMOuter membrane protein Bartonella henselae str. Houston-1, (75.3%)
Iron-regulated outer membrane protein FRPBBMEII010572.05TonB-dependent receptor Plug Domain, OMTonB-dependent receptor Escherichia coli, (41.3%)
Metal chelate outer membrane receptorBMEI065764.80TonB-dependent receptor Plug Domain, OMChain A, Outer Membrane Cobalamin Transporter (Btub) Escherichia coli, (46.2%)
Sugar-binding proteinBMEII059043.20Bacterial sugar-binding extracellular protein, PProbable sugar ABC transporter substrate binding protein Rhizobium etli, (75.7%)
Outer surface proteinBMEII037631.55Surface antigen, OMProbable heat-resistant agglutinin 1 protein Rhizobium leguminosarum bv. viciae, (54.9%)
D-Ribose-binding periplasmic protein precursorBMEII043530.99Periplasmic binding protein and sugar-binding domain of LacI family, PPorin Rhizobium leguminosarum bv. trifolii, (59.2%)
Hypothetical protein BMEI0542BMEI054230.04Unknown, ECHypothetical protein Rhizobium sp., (48.3%)
25 kDa outer-membrane immunogenic protein precursorBMEI100725.24Porin type 2, OMHemin-binding C protein Bartonella tricoborum, (58.7%)
25 kDa outer-membrane immunogenic protein precursorBMEI124923.18Porin type 2, OMHemin-binding B protein Bartonella henselae str. Houston-1, (58.7%)
25 kDa outer-membrane immunogenic protein precursorBMEI182924.58Porin type 2, OMHemin-binding C protein Bartonella tricoborum, (45.4%)
25 kDa outer-membrane immunogenic protein precursorBMEI183024.74Porin type 2, OMOuter membrane protein Rhizobium etli, (60.1%)
31 kDa outer-membrane immunogenic protein precursorBMEII084423.27OmpA-like domain profile, OMPorin Rhizobium leguminosarum bv. trifolii, (52.5%)
BP26BMEI053624.77Protein of unknown function (DUF541), PUnknown function protein DUF541 Rhizobium leguminosarum bv. trifolii, (64.9%)
Precursor YBIS proteinBMEI136923.51Domain YkuD, CHypothetical protein Rhizobium leguminosarum bv. viciae, (67.2%)
OmpA family proteinBMEI078622.96OmpA-like domain, OMOmpA family protein Rhizobium leguminosarum bv. viciae, (81.4%)
Hypothetical lipoproteinBMEI078521.91Prokaryotic membrane lipoprotein lipid attachment profile, IMHypothetical protein Rhizobium leguminosarum bv. viciae, (74.7%)
Ribosome recycling factorBMEI082620.66Ribosome recycling factor (RRF-frr), CRibosome recycling factor Bartonella henselae str. Houston-1, (90.3%)
Hypothetical membrane-associated protein (BMEII0692)BMEII069220.42Invasion-associated locus B (IalB) protein, IMInvasion-associated locus B family protein Rhizobium leguminosarum bv. trifolii, (62.4%)
22 kDa outer membrane protein precursorBMEI071719.44Unknown, OMOuter membrane protein putative precursor Rhizobium leguminosarum bv. viciae, (48.6%)
DNA starvation/stationary phase protection protein DpsBMEI198018.25Dps protein family Ferritin-like domain, CDNA starvation/stationary phase protection protein Dps Escherichia coli, (72.5%)
Peptidoglycan-associated lipoproteinBMEI034018.23OmpA family protein, OMOuter membrane lipoprotein Rhizobium etli, (82.6%)
Invasion protein BBMEI158418.03Invasion-associated locus B (IalB) protein, PInvasion-associated locus B protein Bartonella quintana str. Tolouse, (55.4%)
Outer membrane lipoproteinBMEI013517.60Bacterial outer membrane lipoprotein; Omp19, OMOuter membrane lipoprotein Bartonella henselae str. Houston-1, (59.6%)
Chain A, Cu-Zn superoxide dismutaseBMEII058116.07Cu-Zn superoxide dismutase, PCu-Zn superoxide dismutase Escherichia coli, (68.7%)
Thioredoxin C-1BMEI202211.42Thioredoxin active site, CPutative thioredoxin Rhizobium leguminosarum bv. viciae, (88.8%)
Cochaperonin GroESBMEII104710.3910 kDa chaperonin protein Cnp10, CCochaperonin GroES Bartonella tricoborum, (91.8%)
DNA-binding protein HUBMEI08779.07Bacterial histone-like DNA binding protein signature, C DNA-binding protein Rhizobium sp., (83.5%)
Hypothetical lipoproteinUnknown8.286Prokaryotic membrane lipoprotein lipid attachment profile, OM17 kDa surface antigen Rhizobium leguminosarum bv. trifolii, (61.2%)
Hypothetical protein BMEI0287BMEI02878.596Prokaryotic membrane lipoprotein lipid attachment profile, OMHypothetical protein Rhizobium etli, (56.8%)