Adaptive Immune Response to Model Antigens Is Impaired in Murine Leukocyte-Adhesion Deficiency-1 Revealing Elevated Activation Thresholds In Vivo
Figure 1
Defective humoral immune response upon TT in CD18−/− mice. Eight- to twelve-week-old CD18−/− (open symbols) and WT (filled symbols) mice were immunized intraperitoneally with 2.0 (squares) or 0.2 Lf (circles) of tetanus toxoid (TT)/alum. Animals were reimmunized with the same dose of the antigen at day 34. For assessment of the primary immune response, sera were collected at days 0, 7, and 14, for secondary immune response at days 34, 42, and 49. Subsequently, sera were diluted 1 : 10, and plated out on TT-coated plates in 1 : 6 dilution steps. Serum titers of anti-TT specific IgG1 were determined from the last dilution step where the optical density was still above the background level of the assay. Bars represent the median of each group. *Indicates a for the marked cohorts at all times points shown, from day 14 on.
