Adaptive Immune Response to Model Antigens Is Impaired in Murine Leukocyte-Adhesion Deficiency-1 Revealing Elevated Activation Thresholds In Vivo
Figure 5
Adaptive immunity is functional upon immunization with NP-TT in CD18−/− mice. Eight- to twelve-week-old CD18−/− (open symbols) and WT (filled symbols) mice were immunized intraperitoneally with 2.0 Lf TT/alum (squares) or 2.0 Lf NP-TT/alum. For measurements of anti-TT IgG1, blood was obtained by bleeding from tail veins. Prior to analysis, sera were diluted 1 : 10, and then plated out on TT-coated plates in 1 : 5 dilution steps. Serum titers of anti-TT-specific IgG1 were determined from the last dilution step where the optical density was still above the background level of the assay. For assessment of the primary immune response, results from sera collected at days 7 and 34 are displayed. Besides, measurements for day 49 are shown, and depict IgG1 titers representative also for further time points assessed during secondary immune responses. Bars represent the median of each group.
