From Murine to Human Nude/SCID: The Thymus, T-Cell Development and the Missing Link

Figure 2

Differential gene expression profile, which modulates the discrete stages of the T-cell development. The lymphoid progenitors, entering into thymus and expressing the markers of HSCs, are primed to Notch and IL-7 signaling until DN1 stage. During the transition DN1/DN2, immature thymocytes lose multilineage potential through the downregulation of genes involved in the differentiation towards other cellular lineages, as PU.1, TAL1, GATA-2, and C/EBPα. At the DN2 stage, Myb, GATA-3, HEBalt, GLI-2, and Bcl-11b are upregulated. At the DN3 stage, the genes required for a proper TCR assembly as Rag-1, Rag-2, and pTα are expressed, thus leading to the β-selection. Following β-selection check-point, DN4 cells are fully committed to the TCRαβ ⁺ T-cell lineage.