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Clinical and Developmental Immunology
Volume 2012 (2012), Article ID 478052, 6 pages
Research Article

The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine

1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
2Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
3Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, Fudan University, Shanghai 200032, China
4School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China

Received 20 July 2012; Revised 24 September 2012; Accepted 25 September 2012

Academic Editor: Bapi Pahar

Copyright © 2012 Yanmin Wan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

To confirm the finding that increasing epitope copies could significantly augment the magnitude of specific T cells, 3 mice from each group were randomly selected for IFN-γ+ ELISPOT assay. Our data showed that the frequency of IFN-γ+ T cells could be significantly improved by increasing the epitope copies (P = 0.0007, one way ANOVA, supplementary figure 1). Additionally, a significant linear correlation was observed between the frequency of specific IFN-γ+CD8+ T cells and the epitope copy number (supplementary figure 2).

  1. Supplementary Figure 1
  2. Supplementary Figure 2