Figure 4: Production of cytokines in the intestinal mucosa during the course of intestinal inflammation. Intestines from IL-10−/− at 6, 10 or 16 weeks of age were removed, separated into duodenum, proximal jejunum, distal jejunum, ileum and homogenized in extract buffer. Age-matched 129Sv/Ev (WT) mice were used as controls. Extract supernatant was collected for cytokine assay. IL-17A (a), IL-6 (b), TGF-β (c), and IFN-γ (d) were measured by ELISA. mice per group. Asterisks represent differences among groups in the same intestinal segment (* , ** or *** ).