TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 4
Surface expression of MA3/DP4 TCR on human primary T cells following gene transfer. Human primary T cells transduced with MA3/DP4 TCRαβ genes were stained with TCR-Vβ2 mAb (in which case nonstained MA3/DP4 TCR T cells served as a negative control since control TCRαβ genes also comprise the TCR-Vβ2 chain) (a) or MA3/DP4 tetramer (b) prior to analysis by flow cytometry. In (a), the following T cells were analyzed: parental CD4 T-cell clone R12-C9; TCR T cells, nondepleted (bulk) and TCR T cells depleted for either CD8 or CD4 T cells. These T-cell populations are not FAC sorted. In (b), TCR-transduced T cells, depleted for either CD8 or CD4 T cells nonsorted, or FAC sorted with MA3/DP4 tetramer, were analyzed. Results are from a representative transduction out of 4 transductions of PBMC from 2 donors with similar results.
