Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice

Figure 5

Lysis of B. abortus-infected macrophages by splenocytes from IFN-γ KO and C57BL/6 mice. Macrophages differentiated (5 × 10⁵ cells/well) obtained from IL-12/β2-m^−/−, IFN-γ ^−/−, and C57BL/6 mice were infected with B. abortus (MOI 100 : 1) and used as target cells. Splenocytes (1 × 10⁶ cells/well) obtained from IL-12/β2-m^−/−, IFN-γ ^−/−, and C57BL/6 mice at one week of infection were used as effector cells for cytotoxic assay and were cocultured with macrophages in 24 well plates in DMEM medium. Effector cells were added to target cells in duplicate at 2 : 1 ratio. Significant difference in relation to C57BL/6 and IFN-γ KO mice is denoted by an asterisk (P < 0.05). Results are means ± standard deviations of experiments performed. Data shown are representative of two different experiments.