Polymerized-Type I Collagen Induces Upregulation of Foxp3-Expressing CD4 Regulatory T Cells and Downregulation of IL-17-Producing CD4 T Cells (Th17) Cells in Collagen-Induced Arthritis
Figure 4
Effect of polymerized-type I collagen on the ex vivo intracellular cytokine production and on CD4+ T cell subsets regulation in splenocytes. (a) Spleen cells obtained immediately ex vivo in early arthritis model on day 35 after booster immunization. (b) Splenocytes obtained immediately ex vivo in early arthritis model on day 98 after booster immunization. (c) Spleen cells obtained immediately ex vivo in established arthritis model during first sacrifice. (d) Splenocytes obtained from established arthritis model during second sacrifice. Intracellular production of IL-17A, IL-4, IFN-, and Foxp3 by CD4+ T cells was detected by flow cytometry. Results are representative of 6 mice analyzed in each group. Horizontal dotted line represents mean normal values, obtained from mice () without CIA. Data represent mean ± SEM. *.
