Journal of Immunology Research / 2012 / Article / Fig 2

Research Article

Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma

Figure 2

LIR-1 expression on NK-92 and target cells. Western blot analysis of LIR-1 expression on NK-92 cells and tumor cell lines. Untreated as well as LIR-1 or mock transfected COS-7 cells negative and positive controls, respectively. After film development, the membrane was stripped and reincubated with ACTB antibodies as a loading control. 4  g anti-LIR-1 and 10  g anti-ACTB (1 : 1000) were used for antigen detection. Lanes contended 35  g of total protein. As also shown by flow cytometric analysis, NK-92 as well as IM-9 and MOLP-8 expresses high levels of LIR-1, whereas no detection of LIR-1 was possible on HL60 and K562 cells.

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