Journal of Immunology Research / 2012 / Article / Fig 3

Research Article

Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma

Figure 3

HLA expression. (a). Representative flow cytometric gating strategy for analysis of HLA expression on all used cell lines (shown for RPMI8226). All cells were stained with anti-HLA-I/-E/-G IgG1, followed by secondary goat anti-mouse IgG1 FITC. (b). HLA class I molecules were detectable on all cell lines except for negative control K562. JEG-3 were strongly positive for HLA-6, while detection on NK92 and HL60 was marginal. HLA-E as the ligand for NK62A lacked on all cell linesexcept on minor staining on IM-9.

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