Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
HLA expression. (a). Representative flow cytometric gating strategy for analysis of HLA expression on all used cell lines (shown for RPMI8226). All cells were stained with anti-HLA-I/-E/-G IgG1, followed by secondary goat anti-mouse IgG1 FITC. (b). HLA class I molecules were detectable on all cell lines except for negative control K562. JEG-3 were strongly positive for HLA-6, while detection on NK92 and HL60 was marginal. HLA-E as the ligand for NK62A lacked on all cell linesexcept on minor staining on IM-9.
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