Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
Use of F(ab′)2 does not significantly influence cytotoxicity of NK-92 cells. Statistical analysis (ANOVA) reveals a seemingly protective effect by F(ab′)2 fragments against NK-92 mediated lysis. As influence of the type of antibody and the used concentration were excluded, experimental data were merged. Results were highly significant (untreated ± F(ab′)2/Ab use ± F(ab′)2): IM-9 /; MOLP-8 /; RPMI8226 /; HL60 /; K562 /). The same settings were used throughout all experiments. In the experimental row, K652 tests were performed at the same day and within the same panel. For all other target cells, tests including F(ab′)2 were performed first and were followed by those without additional treatment 1 week later. Though IM-9 renders more susceptible to lysis to a nonsignificant degree, time is the factor most likely to be taken into account, for targets incubated with “untreated” NK cells showed the same changes in sensitivity towards lysis.
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