Journal of Immunology Research / 2012 / Article / Fig 8

Research Article

Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma

Figure 8

Neither antibodies nor F(ab′)2 show toxic effects on NK-92 cells. NK-92 were labeled with 51Cr and incubated with Abs against LIR-1, NKG2A, or IgG2b. Isotype control with (+) or without (−) F(ab′)2 is under the same conditions as the unlabeled effector cells in the blocking experiments. SR is given as mean of four independent experiments for untreated NK cells and as mean of an experimental series with concentration of 10, 1 and 0.1  g/mL. “All Ab mean” integrates those results. F(ab′)2 concentration was 11  /mL. Spontaneous release was <6%. As this control panel was performed in parallel to regular NK-92 blocking experiments, interferences by reagents, temperature, incubation period, cell viability, or technical equipment can be excluded.

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