Involvement of Stat1 in the Phagocytosis of M. avium
Figure 2
Time course of Stat1/p-tyr701Stat1 signalling pathway activation after internalization of live M. avium (a), heat-killed (H.K.) M. avium (b), and latex particles (c), in the different opsonization conditions. The opsonization methods are as follow: human pooled serum (AB), decomplemented human serum (d-AB), and purified human IgG (IgG). (a) Left: figure of western blot of p-tyr701Stat1 and actin levels at 0, 24, 48, and 72 hours after uptake of live M. avium; right: in graph, we report the time-dependent expression of p-tyr701Stat1. The protein expression levels were normalized to the amount of actin and expressed as percentage of control value which was placed as 100%. (b) Left: figure of western blot of p-tyr701Stat1 and actin levels at 0, 24, 48, and 72 hours after phagocytosis of heat-inactivated M. avium; right: in the graph, we report the time-dependent expression of p-tyr701Stat1. The protein expression levels were normalized to the amount of actin and expressed as percentage of control value which was placed as 100%. (c) Left: figure of western blot of p-tyr701Stat1 and actin levels at 0, 24, 48, and 72 hours after internalization of latex particles; right: in the graph, we report the time-dependent expression of p-tyr701Stat1. The protein levels were normalized to the amount of actin and expressed as percentage of control value which was placed as 100%. The results are the mean of three different experiments. Statistical analyses were performed with Student’s t-test (Prism 4 Graph Pad software). (#) significant difference from control (CTR) .
