Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants

Figure 1

Percutaneous gastroduodenojejunostomy to deliver FDS or vehicle directly to the jejunal mucosa of Lewis rats. (a) Midline laparotomy. (b) Silicone tubing was delivered through the anterior abdominal musculature into the stomach and passed distal to the Treitz ligament. (c) The abdomen was closed and the tubing secured to abdominal wall musculature and tunneled to the posterior neck. (d) The tube was additionally secured at the posterior neck and sealed with a stopper that could be removed and replaced for intrajejunal administrations. (e) Characteristic jejunal histological appearance of intestinal tissue 5 mm distal to the tube end at time of sacrifice confirmed correct intrajejunal placement in all recipients.