Figure 3: (a and b): Surface expression of E-cadherin on T3 M4 or HuH7 was determined by cytofluorometry (left panels). The manually dispersed cells without treatment (w/t), cells co-cultivated with PMN (PMN) or PMN-derived elastase (Ela) for 3 h are shown (the vertical line shows the position of the end of the IgG-isotype control). On the right panels, the respective Western blots are shown. (c and d): Expression of keratins was determined by cytofluorometry with permeabilised cells (untreated, or co-cultivated with PMN-elastase or PMN for 24 h or by western blotting).