Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase

Table 2

PMN and PMN-elastase cause dyshesion, loss of E-cadherin expression, and downmodulation of keratin in cancer cells.

𝑛=3	T3M4		HuH7
Cultivated with	PMN	PMN elastase	PMN	PMN elastase
Dyshesion*	47,19% (±2,30)	38,86% (±7,33)	37,05% (±1,91)	46,33% (±3,84)
E-cadherin expression**	53,81 (±20,22)	78,76% (±19,64)	22,61% (±5,55)	54,12% (±23,04)
Keratin expression	36,78% (±20,13)	31,85% (±20,18)	17,57% (±9,56)	37,20% (±28,77)

*Measured as area devoid of cells in relation (%) of the total cell area.
**Measured as mean fluorescence intensity (MFI) in relation to the MFI of untreated cells which were set as 100 %.