Figure 2: Regulatory T cells in lymphoid organs. Frequency of Foxp3+CD4+ T cells in (a) spleen, (b) cervical lymph nodes (cLN), and (c) mesenteric lymph nodes (mesLN) of C57BL/6 fed or not with OVA before and after OVA/Alum sensitization. Cells recovered from the different lymphoid organs were stained for CD4 and Foxp3 and gated in CD4-positive cells. Values are representative of two independent experiments with pooled cells from three animals per group.