Figure 4: Airway regulatory T cells from allergic mice express a memory/effector phenotype. (a) FACS-Histograms of CD4+Foxp3+ cells from allergic mice expressing CCR4, CCR7, CD62L, CD54, CD44, CTLA-4, GITR, CD25 in BAL (red line), or mediastinal draining lymph nodes (dLN) (blue line). The numbers into each histogram represent the mean fluorescence intensity (MFI). Kinetic of lung CD4+CD69+ cells frequency expressing or not Foxp3. (c) Percentage of BAL CD4+CD69+ cells expressing or not Foxp3. Pooled cells from four mice recovered from lung or BAL were stained for CD4, CD69, and Foxp3 and gated in CD4-positive cells. The results are representative of two experiments with four mice per group.