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Figure 6: Lung CD4+CD25+ T cells from allergic mice suppress T-cell proliferation but not Th2 cytokine production. (a) Proliferation of MACS-purified lung CD4+CD25 or CD4+CD25+ cells from BALB/c allergic mice alone or cocultured at different CD25/CD25+ ratios determined by 3H-thymidine (3H-TdR) incorporation after anti-CD3 stimulation. ELISA assays for (b) IL-4 and (c) IL-5 in the culture supernatants showed in (a). Values represent the means ± SEM of triplicate wells. The results are representative of two experiments. Significant differences * , ** , and *** are shown.