Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production

Figure 6

Lung CD4⁺CD25⁺ T cells from allergic mice suppress T-cell proliferation but not Th2 cytokine production. (a) Proliferation of MACS-purified lung CD4⁺CD25⁻ or CD4⁺CD25⁺ cells from BALB/c allergic mice alone or cocultured at different CD25⁻/CD25⁺ ratios determined by ³H-thymidine (³H-TdR) incorporation after anti-CD3 stimulation. ELISA assays for (b) IL-4 and (c) IL-5 in the culture supernatants showed in (a). Values represent the means ± SEM of triplicate wells. The results are representative of two experiments. Significant differences *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 are shown.

(a)

(b)

(c)