Tumor-Induced CD8+ T-Cell Dysfunction in Lung Cancer Patients
Figure 2
Pleural effusion, but not peripheral blood, CD8+ T-cells from lung cancer patients are sensitive to AICD. Both pleural effusion and peripheral blood CD8+ T-cells from lung cancer patients (n = 10) were stimulated with anti-CD3 mAbs for 24 h; then apoptosis was determined by annexin V binding in activated CD8+CD69+ T-cells by multiparametric flow cytometry; propidium iodide was used to exclude necrotic cells. (a) Results from a lung cancer patient. (b) Anti-CD3 induced apoptosis was determined as described in [101], comparison was made by paired Student’s -test. Bars depict the mean ± Standard Error.
