Journal of Immunology Research / 2012 / Article / Fig 1

Research Article

Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells

Figure 1

Different maturation stages of NK cells according to expression levels of CD27 and CD11b. Flow cytometry analysis was performed to analyse lymphocytes from the liver and spleen of B6 mice at the ages of E20, the neonatal stage, and at 3 days, 1 week, 3 weeks, 4 weeks, 6 weeks, 8 weeks, and 10 weeks, which were stained with the indicated antibodies. NK cells (CD3NK1.1+) were gated to analyse the expression of CD27 and CD11b. Six to seven fetal mouse livers or spleens were put together to acquire enough cells to perform FACS analysis in one experiment, and three independent experiments were performed. In the other groups, there were three mice independently detected for one experiment, and three independent experiments were performed. (a) The percentages represent the net percentage (%) of cells in the appropriate quadrant. These are from a single experiment representative of three independent experiments. (b) The percentages of four NK cell subsets in the liver and spleen were calculated. Data are shown as the mean ± SEM from three mice in each group. *** , ** , * .

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