Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
Figure 2
Different expression patterns of the NKG2A/Ly49 family of receptors on NK cells during mouse ontogeny. Flow cytometry was performed to analyse the lymphocytes stained with indicated antibodies from liver and spleen of B6 mice at E20, the neonatal stage, and at 3 days, 1 week, 3 weeks, 4 weeks, 6 weeks, 8 weeks, and 10 weeks. NK cells (CD3−NK1.1+) were gated to analyse the expression of NKG2A and Ly49. In each independent experiment, six to seven fetal mouse livers/spleens were put together to acquire enough cells to perform FACS analysis, and independent experiments were repeated for three times. (a) The percentages represent the net percentage (%) of cells in the appropriate quadrant. These are from a single experiment representative of three independent experiments. (b) The percentages of the NKG2A+ NK cell subset (%) and the Ly49+ NK cell subset (%) were calculated from the total number of NK cells in the liver and spleen. Data are shown as the mean ± SEM from three mice in each group. ***, **, * compared with the corresponding group.
