Journal of Immunology Research / 2012 / Article / Fig 3

Research Article

Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells

Figure 3

Stimulatory receptors expressed on NK cells during mouse ontogeny. At the indicated time points, mononuclear cells were isolated from liver and spleen and analysed by flow cytometry for the expression of stimulatory receptors. NK cells (NK1.1+CD3) were gated, and each marker was analysed by histogram. Unstained controls are shown in grey. The expression of NKG2D (a), NKp46 (b), 2B4 (c), and CD2 (d) on liver NK cells is shown and compared with spleen NK cells during ontogeny. In each independent experiment, six to seven fetal mouse livers or spleens were put together to acquire enough cells to perform FACS analysis in one experiment, and three independent experiments were performed. In the other groups, there were three mice independently detected for one experiment, and three independent experiments were performed. The mean fluorescence intensity (MFI) and positive percentage of each marker are shown as mean ± SEM from three mice in each group. These are from a single experiment representative of three independent experiments. *** , ** , * compared with the corresponding group.

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