Fibronectin Binding Is Required for Acquisition of Mesenchymal/Endothelial Differentiation Potential in Human Circulating Monocytes
Figure 2
Proliferative capacity and gene expression profiles of adherent cells obtained in the MOMC generation cultures on fibronectin or collagen (I). (a) PBMCs () were cultured on 24-well plastic plates coated with fibronectin (Sigma) or collagen (I). The medium containing floating cells was exchanged with fresh medium every 3 days. At day 7, all adherent cells were detached, counted, and replated on plates coated with fibronectin (Sigma) or collagen (I). Results are expressed as proportion of the number of adherent cells recovered at each passage to the number of adherent cells recovered at the first passage. The mean and standard deviation of 5 independent experiments are shown. (b) Adherent cells recovered were subjected to reverse transcription and semi-quantitative PCR for evaluation of mRNA expression. Positive controls for the specific amplification included pooled PBMCs for CD14, CD45, and GAPDH; cultured human umbilical vein endothelial cells for CD34, CD105, and CD144; cultured skin fibroblasts for collagen (I), collagen (III), and fibronectin. The results are representative of 4 independent experiments.
