Fibronectin Binding Is Required for Acquisition of Mesenchymal/Endothelial Differentiation Potential in Human Circulating Monocytes
Figure 4
Effects of anti- integrin mAb, RGD domain peptide, or CS-1 domain peptide on MOMC generation. (a) MACS-sorted CD14+ monocytes () were cultured with PBMC-conditioned medium on 24-well plastic plates coated with fibronectin (Sigma) for 7 days. Anti- integrin, anti- integrin, or anti- integrin mAb was added at the initiation of cultures and added again every other day. The medium containing floating cells was exchanged with fresh medium at day 3. Adherent cells were observed with an inverted microscope. Bars: 100 μm. (b) An RGD domain peptide (GRGDSP) or its corresponding control peptide (GRGESP) or a CS-1 domain peptide (EILDV) or its corresponding control peptide (EILAV) was added at the initiation of cultures and added again every other day. The number of adherent cells obtained at day 7 was counted. The results are expressed as proportion of the number of adherent cells in individual cultures to the number of adherent cells in the control culture without peptide or vehicle. The results shown are the mean and standard deviation of 3 independent experiments.
