Fibronectin Binding Is Required for Acquisition of Mesenchymal/Endothelial Differentiation Potential in Human Circulating Monocytes

Table 1

Primer sequences and PCR conditions.

Gene		Primer sequences (5′ to 3′)	Annealing temperature (°C)	PCR cycles
AML-1	Sense	ACAAGGCAGATCCAACCATC	63	33
	Antisense	GTCGGGGAGTAGGTGAAGG
c-Myb	Sense	CTGCTCACACCACTGGGAAG	63	38
	Antisense	CGAGCTTGACTGGAAGATGTC
Egr-1	Sense	TGACCGCAGAGTCTTTTCCT	63	35
	Antisense	GATGAGCTGGGACTGGTAGC
Foxp1	Sense	ACGCCTACTGCACACCTCTC	63	30
	Antisense	CTTCAGCTTCCTCTGGATCG
IRF-7	Sense	ACTGTGACACCCCCATCTTC	63	30
	Antisense	GCTCCATAAGGAAGCACTCG
PU.1	Sense	AGATGCACGTCCTCGATACC	63	30
	Antisense	GCTTGGACGAGAACTGGAAG

AML-1: acute myelogenous leukemia-1, Egr-1: early growth response-1, IRF-7: interferon regulatory factor-7.