Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3

Figure 5

(a-b) Western blotting with sera to P1 or P2. Lane 1, prestained protein marker; Lanes 2 and 3, induced and uninduced GST-hZP3^172-190; Lanes 4 and 13, induced hZP3b^177-348; Lanes 5 and 6, induced Stv108 and GST188; Lanes 7 and 8, induced and uninduced GST-hZP3^301-320; Lanes 9 and 10, induced and un-induced GST-P37; Lanes 11 and 12, induced and un-induced GST-P44. (c–f) matching light images for the human oocytes imaged by immunofluorescence in panels g–j. (g–j) reactivity of rabbit sera to P1 or P2 with human oocyte by indirect immunofluorescence. Representative immunofluorescence patterns are shown for (g) preimmune serum; (h) immune serum only received CFA (i-j) immune serum to P1 or P2.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)