Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates

Table 2

Summary of rabbit immunogenicity studies with different EV71 vaccine candidates.

EV71 vaccine candidates	Adjuvant formulated	Total IgG titers		Western blot			Virus neutralization titer
		VP1-43	VP2-28	rVP1	rVP2	rVP3	EV71 (B4 E59)	CVA16 (N5079)
2 μg of inactivated
EV71 virion	PBS	1/200	1/800	+++	+++	++	1/128	<1/8
EV71 virion	Alum	1/200	1/6400	+++	+++	++	1/6400	1/32
5 μg of inactivated
EV71 virion	Alum	1/200	1/6400	+++	+++	+++	1/12800	1/64

Two to three rabbits per group were immunized 3 times with EV71 vaccine candidates alone or formulated with alum. The protocols for IgG titer determination, western blot analysis, and virus neutralization assay are described in Section 2.