The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells

Figure 2

PPARγ1 and PPARγ2 mRNA expression. (a) PPARγ1 and (b) PPARγ2 gene expression levels in unstimulated (open bar, 𝑛=13) and stimulated (grey bar, 𝑛=13) naïve and memory CD4+ T cells or those treated with ciglitazone (solid bar, 𝑛=8). The PPARγ1 and PPARγ2 gene expression levels were calculated as the number of mRNA transcripts per μg total RNA. The data plotted is the mean mRNA transcripts ± SEM. Statistical analyses were performed using the Mann-Whitney U test. *𝑃<0.05; **𝑃<0.01—significantly different from unstimulated cells. ^#𝑃<0.05; ^##𝑃<0.01—significantly different from CD3/CD28–stimulated cells. ^¶𝑃<0.05—significantly different from correspondingly treated CD45RA+ cells.

(a)

(b)