The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells

Figure 4

Inflammatory cytokine expression. Relative mRNA expression levels of selected cytokine genes in naïve and memory CD4+ T cells following CD3/CD28 stimulation in the presence or absence of ciglitazone (𝑛=5). Untreated cells (open bar), stimulated cells (grey bar), and ciglitazone-treated cells (solid bar) were assessed for their relative expression of (a) TGFβ, (b) IL-1β, (c) IL-8, (d) TNFα, (e) GM-CSF, and (f) IL-6, as a ratio of GAPDH. Statistical analyses were performed using the Mann-Whitney U test. *𝑃<0.05, **𝑃<0.01. ^#Significance levels cannot be analyzed because the gene expression was not detectable.

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