Inhibition of Arterial Allograft Intimal Hyperplasia Using Recipient Dendritic Cells Pretreated with B7 Antisense Peptide
B7AP-pretreated DCs inhibited T-cell proliferation in MLR. Under high concentrations of B7AP (100 mg/L), T cells demonstrated a low proliferation capacity. However, proliferation of T cells surged to a rather high level when the final concentration of B7AP went down from 10 mg/L to 1 mg/L, followed by a plateau (a), indicating that B7AP final concentration of 10 mg/L could ensure maximal blockage of DC surface B7 molecules. The BM-DCs were pretreated by the B7AP and control peptide at the same concentration (10 mg/L), and the MLR was performed as described above (b). NS: normal saline group, MYPPPY group: relative peptide, FTD10 (FTDSVRDPKT) group: negative control peptide.
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