Association between Functional Polymorphisms of Foxp3 Gene and the Occurrence of Unexplained Recurrent Spontaneous Abortion in a Chinese Han Population

Table 1

Primers used in the genotyping by PCR-SSP or PCR-RFLP.

Marker	Allele	Forward primer	Reverse primer	PCR product Tm/°C
Method	Enzyme
rs2232365	A	5′-CCCAGCTCAAG	5′-GGGCTAGTGAG	442 bp
PCR-SSP		AGACCCCA-3′	GAGGCTATTGTAA C-3′
	G	5′-CCAGCTCAAGA	5′-GCTATTGTAACA	427 bp
		GACCCCG-3′	GTCCTGGCAAGTG-3′
rs5902434	deletion	5′-ACCTTTAAGTCTTCTGCC	5′-TGATTATCAGCG	356 bp
PCR-SSP		ATTTATTCTATTATTT-3′	CACACACTCAT-3′
	ATT	5′-CCTTTAAGTCTTCTGCCA	5′-TGATTATCAGCG	358 bp
		TTTATTCTATTATTA-3′	CACACACTCAT-3′
rs3761548	PstI	5′-GCCCTTGTCTA	5′-CAGCCTTCGCCA	487 bp
PCR-RFLP		CTCCACGCCTCT-3′	ATACAGAGCC-3′	63°C
rs2294021	HaeIII	5′-CACACACAATCCAT	5′-ATCTCCATGCCCTA	429 bp
PCR-RFLP		CCCAGTCACCC-3′	AGAAGGCCACC-3′	59°C