PD-L1-Expressing Dendritic Cells Contribute to Viral Resistance during Acute HSV-1 Infection
Figure 5
Functional analysis of MLN CD11c+ cells following HSV-1 infection and treatment with anti-PD-L1 or anti-PD-L2 antibody. C57BL/6 mice were infected with 1,000 PFU HSV-1/cornea. At days 2, 4, and 6 p.i. mice were administered neutralizing antibody to PD-L1, PD-L2, or control IgG. At day 7 p.i. the mice were exsanguinated and CD11c+ cells were highly enriched from the MLN. CD11c-enriched MLN cells were pulsed with UV-inactivated HSV-1 and cocultured with HSV gB-specific T-cell receptor T cells of transgenic mice. Following incubation, the supernatants were removed and assayed for IL-2 (a), IL-10 (b), and IFN-γ (c) levels by suspension array. Background cytokine levels were determined using nonpulsed CD11c+-enriched MLN cells. Background levels of cytokine production were subtracted from corresponding UV-inactivated HSV-1-pulsed samples. This figure summarizes two experiments (). Each bar represents the mean ± SEM. * comparing the indicated group to the isotypic control antibody-treated group.
