IgG Placental Transfer in Healthy and Pathological Pregnancies

Figure 4

Immunoblotting of anti-EHEC O157:H7 IgG antibodies in the paired maternal and cord samples. Bacterial proteins were separated by 12.5% SDS-PAGE. Paired maternal and cord serum samples are identified numerically. M: maternal serum; C: cord serum; NHS: pool of healthy adult serum samples (normal human serum). The immunoblots were developed with antihuman IgG conjugate. Molecular weight standards are on the left for samples 1–8 and on the right for the pool of normal human serum. It was observed that there is almost complete identity between the antigens recognized by maternal and umbilical cord sera [58].